Originally Posted by thegenemaster

It takes me about 4-6 hours to isolate the DNA from 96 plants. Next day I set up the reactions. I have them set up as a master mix. I add 1ul of isolated DNA to each reaction and add 39up of my master mix that I have determined experimentally will work every time. I can do 96 reactions at once in my thermocycler. Run them out on 3-4 gels for an hour. Take a photo and I'm done. Ten dollars a plant is a very reasonable rate and at that rate I make just under $1000 for less than a days work. I have an abundance of enzyme and my specific choice of forward and reverse primers so I can do literally thousands of reactions. I only have to pay for pipet tips and microfuge tubes. Both of which are cheap if you buy them in bulk.