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Thread: How to Make Female Seeds

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    How to Make Female Seeds

    first off, i am a former grower who just joined up here. i did not author this but i used to use this method and it works...

    thought i would share since i could not find this info when searching here. if it has already been posted forgive me but i couldn't find it...

    The mix is called STS (Silver Thiosulfate Solution)

    420 Magazine - View Single Post - Any1 know how to change a male plant to female?

    Yes Boss, you are correct, but I have tried to do male to female, and I used STS.
    Here is my "Juice" for creating male flowers on a female plant. I use a Sliver Thiosulphate solution.

    1. mix 2.6 grams of sodium thiosulphate into 600ml of distilled water and mix thoroughly.

    2. mix .7 grams of silver nitrate into 100ml of distilled water and mix thoroughly.

    3. mix silver nitrate solution into sodium thiosulphate solution while stirring rapidly.

    4. top off mixture with distilled water to fill a glass quart jar (canning jar type).

    5. use this mix at a 3:1 ratio with distilled water and spray plants to point of run-off.

    6. repeat process in 3 days, move target females in at 5 days.

    use common safety precautions. rubber gloves, safety glasses, skin covered, respirator preferred.

    3:1 ratio of distilled water to stock mix of STS can be played with some. some people use it as diluted as 9:1 and some run it as hot as 1:1.


    This may be helpful as well.

    How To Reverse Sex Using Silver Thiosulfate Solution

    The following is a safe, inexpensive, and successful method for reversing the sex of female cannabis plants. Individual plant responses may vary based upon strain, but I can verify that this process is fully effective in stimulating profuse staminate flower production.

    This process can be used to:

    A: create new feminized seeds from solitary prize mothers that you currently have
    B: create interesting feminized-seed hybrids from different prize strains that you currently have
    C: create feminized seeds for optimum outdoor use
    D: accelerate the "interview" phase of cultivation, in searching for interesting new clone-mothers
    E: reduce total plant numbers- great for medical users with severe plant number restrictions
    F: increase variety, by helping to create stable feminized seedlines to be used as an alternative to clones

    At the bottom of this post are some specific details about the chemicals used, their safety, their cost, and where to get them.

    It is important to educate yourself about cannabis breeding theory and technique prior to using a method like this one. Here is a link to Robert Clarke's "Marijuana Botany", which is a very good reference.

    "Marijuana Botany" by Robert Connell Clarke
    (unfortunately missing the appendices)
    Marijuana Botany

    It is also important to use basic safety precautions when mixing and handling these chemicals, so read the safety data links provided. The risk is similar to mixing and handling chemical fertilizers, and similar handling procedures are sufficient.

    Remember: nothing will ever replace good genetics, and some of your bounty should always go back towards the professional cannabis breeders out there... the ones who have worked for many generations to come up with their true-breeding F1 masterpieces. Support professional breeders by buying their seeds.

    ************************************************** ********
    First, a stock solution is made. It consists of two parts (A and B) that are initially mixed separately, then blended together. Part A is ALWAYS mixed into part B while stirring rapidly. Use distilled water; tap water may cause precipitates to form.

    Wear gloves while mixing and using these chemicals, and mix and use in a properly ventilated area. A mask will prevent the breathing of any dust, which is caustic. STS is colorless and odorless, and poses minimal health risks if used as described here. (See material safety data sheet links below). Note that silver nitrate and STS can cause brown stains upon drying, so spray over newspaper and avoid spilling.

    Part A: .5 gram silver nitrate stirred into 500ml distilled water
    Part B: 2.5 grams sodium thiosulfate (anhydrous) stirred into 500ml distilled water

    The silver nitrate dissolves within 15 seconds. The sodium thiosulfate takes 30-45 seconds to dissolve.

    The silver nitrate solution (A) is then mixed into the sodium thiosulfate solution (B) while stirring rapidly. The resulting blend is stock silver thiosulfate solution (STS).

    This stock solution is then diluted at a ratio of 1:9 to make a working solution. For example, 100ml of stock STS is added to 900ml of distilled water. This is then sprayed on select female plants.

    Both the stock STS and the working solution should be refrigerated after use, as well as the powdered chemicals, to avoid activity loss. Excess working solution can be safely poured down the drain after use (with ample running water) with negligible environmental impact. It's pretty cheap.

    Each liter of stock STS will make ten 1-liter batches of working solution of STS. With the minimum amount of base chemicals ordered from Photographer's Formulary (see link below), this means that each 1-liter bottle of working solution STS costs less than 9 cents, and can treat 15-20 mid-sized plants. That's 200 1-liter batches of STS for $18. Note that the distilled water costs far more than the chemicals.

    The STS working solution is sprayed on select female plants until runoff. Do the spraying over newspaper in a separate area from the flower room. You probably won't smell anything, but ventilate anyway. You now have what I call a "F>M plant"; a female plant that will produce male flowers.

    After the F>M plant dries move it into 12/12 immediately. This is usually done three to four weeks prior to the date that the target (to be pollinated) plants will be ready to pollinate. Response times may vary slightly depending upon the strain. More specific times can be determined by trial with your own individual strains. In my trials it took 26 days for the first pollen. 30-35 days seems optimum for planning purposes.

    So, assuming that a target plant needs 3-4 weeks to produce fully mature seeds, a strain that takes 8 weeks to mature should be moved into flower at about the same time as the female>male plant. A target plant that finishes flowering in 6 weeks needs to be moved into flower later (10 days or so) so that it doesn't finish before the seeds can fully mature.

    A seeded individual branch can be left to mature on a plant for a bit longer, while harvesting the other seedless buds if they finish first. Just leave enough leaves on for the plant for it to stay healthy.

    Effects:

    Within days I noticed a yellowing of the leaves on the F>M plants. This effect persisted for two weeks or so; after this they became green again, except for a few of the larger fans. The plants otherwise seemed healthy. No burning was observed. Growth stopped dead for the first ten days, and then resumed slowly. No stretch was ever seen. After two weeks the F>M plants were obviously forming male flower clusters. Not just a few clusters of balls, but complete male flower tops. One plant still formed some pistillate flowers, but overall it was predominantly male.

    It is strange indeed to see an old girlfriend that you know like the back of your hand go through a sex change. I'll admit that things were awkward between us at first.

    When the F>M plants look like they may soon open and release pollen, ( 3-1/2 to 4 weeks) move them from the main flower room into another unventilated room or closet with lighting on a 12/12 timer. Don't worry too much about watts per square foot; it will only be temporary.

    When the pollen flies, move your target plants into the closet and pollinate.

    A more controlled approach is to isolate the F>M plants in a third remote closet (no light is necessary in this one, as they are releasing pollen now and are nearly finished anyway). In this remote other closet the pollen is very carefully collected in a plastic produce bag or newspaper sleeve and then brought back to the lighted closet, where the target plants are now located. If this is done, be careful to not mix pollen types by letting the F>Ms dust each other. Avoid movement, or use yet another closet.

    Take special care to not let pollen gather on the outside of this bag- a static charge is sometimes present. Drop small open clusters of blooms inside and then close the bag at the mouth and shake. Important: next, step outside and slowly release the excess air from the bag, collapsing it completely, so that pollen doesn't get released accidently. Point downwind; don't let it get on your hands or clothes.

    This collapsed pollinated bag is now very carefully slipped over only one branch and is then tied off tightly at the mouth around the branch stem with a twist tie or tape, sealing the pollen inside. Let the bag inflate slightly with air again before sealing it off, so the branch can breathe. This technique keeps the entire plant from seeding. Agitate the bag a bit after tying it off to distribute the pollen. Don't forget to label the branch so you know which seeds are which. Other branches on this same plant can be hit with different pollen sources.

    If no lighted closet is available, the plant can be moved back into the main room, but- be very careful: pollen is sneaky. After 4-5 days, the bag is gently removed and the plant completes it's flowering cycle.

    Yet another method has worked well for me. I position the target plants in a non-ventilated lighted closet, and then I collect pollen on a piece of mirror or glass. This is then carefully applied to the pistils of one pre-labeled branch by using a very fine watercolor paintbrush. Care is taken to not agitate the branch or the pollen. No sneezing. The plant needs to be in place first; moving it after pollination can shake pollen free and blow this technique.

    Regardless of technique, at completion you will have feminized seeds. Let them dry for 2-4 weeks.

    Silver nitrate is a white crystalline light-sensitive chemical that is commonly used in photography. It is also used in babies' eyes at birth to prevent blindness. It can cause mild skin irritation, and it stains brown. Avoid breathing. I didn't notice any smell or fumes, but ventilation is recommended. Be sure to wash the spray bottle well before you use it elsewhere; better yet: devote a bottle to STS use. A half gram is a surprisingly small amount; it would fit inside a gel capsule.

    Here are links to some safety data. A Google search will bring up more information if needed.

    Silver Nitrate info:
    ICSC:NENG1116 International Chemical Safety Cards (WHO/IPCS/ILO) | CDC/NIOSH
    http://www.lions.odu.edu/~redwards/...%20solution.pdf

    For a realistic hazard level comparison, here is a link for the safety and handling data for Ammonium Nitrate, or common fertilizer:
    Page Not Found Error

    Sodium thiosulfate is also a white crystalline chemical commonly used in photography; it is used in photographic fixers. Same general cautions apply, minus the staining. This formula uses the anhydrous type. Non-hazardous.

    Sodium Thiosulfate info:
    http://ptcl.chem.ox.ac.uk/MSDS/SO/s...hiosulfate.html
    http://www.med-chem.com/MSDS/Sodium_Thiosulf.htm

    For what it's worth, here is some information I found about sex-reversal in cannabis. This was printed in the memoirs of H.Y. Mohan Ram, found as a .pdf file which I discovered by searching google for "Mohan Ram and Sett" as cited by Vic High on this board.
    Here is a link to the .pdf file: http://www.ias.ac.in/jbiosci/dec2002/651.pdf -- I enjoyed reading this man's words, he seems like a wonderful and wise human being.

    He states that fertile male flowers can be induced in female cannabis plants using:
    gibberellins (GAs) and anti-ethylene agents such as:
    silver nitrate (AgNO3)
    silver thiosulphate anionic complex (STS)
    aminoethoxyvinyl glycene (AVG)
    and cobalt chloride (CoCl2).

    As this was an aside mentioned in the larger context of Dr. Ram's life work, he does not go into detail regarding the methods of application of these chemicals to achieve the sex reversal. I'm sure the papers Vic mentioned will give the specifics, here they are again (thanks Vic):

    Mohan Ram H Y and Juiswal VS. 1972. Induction of male flowers on female plants of Cannabis sativa by gibberellins and its inhibition by abscisic acid. Plants, 105:263-266.

    Mohan Ram H Y and Sett R. 1981. Modification of growth and sex expression in Cannabis sativa by aminoethoxyvinylglycine and ethephon. Z. Pflanzenphysiol., 105:165-172.

    Mohan Ram H Y and Sett R. 1982. Induction of fertile male flowers in genetically female Cannabis sativa plants by silver nitrate and silver thiosulphate anionic complex. Theor. Appl. Genet., 62:369-375

    Source: Saskafarian



  2. #2
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    oh well, it does work. just thought i would post it here for some to try

  3. #3
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    -Or-
    you can add a couple of uncoated aspirin to a quart of water. A couple or few applications during normal watering schedule, and you'll get the nanners.

    -Or-
    you can take one female out of her flowering closet a few nights in a row. Put her under a couple of CFL's for 15 minutes a night, returning her to her flower closet in the morning. Nanners will form. I use this technique most often. No worries about smoking additional residue.

    I'm not a big fan of those extremely invasive methods of stressing the ladies, and it renders the plant potentially toxic for smoking. I've never seen a study that has tested the effects of smoking steroids, hormones or gibberellins, or their respective vapors, but would not take the use of these chemicals/hormones/steroids on our smokables, lightly.
    Since rarely does someone cull the pollen donor, (the one that has been poisoned) it's usually just smoked or sold. (dispensaries)

    Gibberellins and heavy metals just shouldn't be smoked and really have no place in a medical-users stash box. Especially if drug interractions are a concern, as some of us are on a variety of medications and treatments.

    Doubtful it's doing cannabis genetics any favors, either.
    (Don't fem a femmed lady. Mutants are possible, if not likely.)

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    Quote Originally Posted by Rusty Trichome View Post
    -Or-
    you can add a couple of uncoated aspirin to a quart of water. A couple or few applications during normal watering schedule, and you'll get the nanners.

    -Or-
    you can take one female out of her flowering closet a few nights in a row. Put her under a couple of CFL's for 15 minutes a night, returning her to her flower closet in the morning. Nanners will form. I use this technique most often. No worries about smoking additional residue.

    I'm not a big fan of those extremely invasive methods of stressing the ladies, and it renders the plant potentially toxic for smoking. I've never seen a study that has tested the effects of smoking steroids, hormones or gibberellins, or their respective vapors, but would not take the use of these chemicals/hormones/steroids on our smokables, lightly.
    Since rarely does someone cull the pollen donor, (the one that has been poisoned) it's usually just smoked or sold. (dispensaries)

    Gibberellins and heavy metals just shouldn't be smoked and really have no place in a medical-users stash box. Especially if drug interractions are a concern, as some of us are on a variety of medications and treatments.

    Doubtful it's doing cannabis genetics any favors, either.
    (Don't fem a femmed lady. Mutants are possible, if not likely.)

    i've never smoked one of the plants i sprayed with the STS. always just used it for see making..

    thanks for the other tips...

  5. #5
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    Ya Rusty, I wouldn't smoke anything I put that kind of garbage on, much less a male, it just doesn't get that good. So if it goes male it is trash.

    I will try that aspirin process on that one I cannot get to produce banannas. I have 4 crosses from that herm plant, so do have some new stuff to test out in the meantime.

    Very interesting topic tho. One sure can come up with some neat crosses.

    I prefer to gather my pollen by getting the nanners just before the open, and I have these little plastic containers with slip lids. Put them in there and they open and the pollen stays put. When I want to breed a plant I just shake the little container, get the dust on the clear lid and then settle that down over the bud I am breeding, smush it around so the pollen is stuck on, mark the bud and forget it. Seems to not effect any others yet.
    Natural Spider Mite Control
    Trouble shooting form

    Thank you Lord for this green thumb, I so love to grow your gift of cannabis.

  6. #6
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    Uncoated and made from this active ingredient:
    Salicylic acid
    (may as well learn a little about while you're there, lol)

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