I've got a eCup test coming up tomorrow. I will have been clean for 23 days and want to get your opinion on whether I should go with substitution or dilution. My first instinct is both, in case something goes wrong (temp isn't right, etc.). However, the only person I know I can get a clean sample from is my pregnant wife. I'm hesitant to use her sample, not because she's pregnant or she's a female, but that I've read pregnant women have much lower creatine (or creatinine) levels than normal. I've been taking a creatine supplement since yesterday, but don't want to have her take any (it's really too late anyway from what I understand). And I've never subbed before, so it makes me nervous as hell. I've got temp strips and am getting a bottle today to use for subbing, but would feel so much better if I could be confident in diluting. Check my results in the attachment and let me know what you think. I know a line is a line is a line, but if the attendant has poor eyesight, they might not see this line. This comes from my first void of the morning. I plan on testing my dilution method (as per the sticky, minus the aspirin, plus the gatorade) and re-test. If it comes up neg, should I be okay? The test is at a doctor's office, not a corporate lab like Quest (I don't know if that makes a difference). Gotta get this job. I sincerely appreciate any help you all can offer. Thank you, this board has a huge amount of helpful information.